Journal: Materials Today Bio

Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer

doi: 10.1016/j.mtbio.2026.102835

Figure Lengend Snippet: (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.

Article Snippet: Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China).

Techniques: Staining, Fluorescence, Imaging, Expressing, Western Blot

Journal: ACS Nano

Article Title: Drug-free Immunotherapeutic Biomimetic Nanoparticles for Treating Triple-Negative Breast Cancer

doi: 10.1021/acsnano.5c18774

Figure Lengend Snippet: MPsomes reduced macrophage migration more effectively than liposomes in a Transwell static assay. (A) Schematic representation of the Transwell macrophage migration assay model used for inhibition evaluation. (B) The number of migrating macrophages in the Transwell inserts for each treatment group [a.u.]. (C) Percentage of the area occupied by migrating macrophages within the whole insert area (5.5 mm × 5.5 mm) for each treatment group. Each point represents an independent insert, quantified using image analysis tools. (D) Representative images of stained macrophages at the bottom of the Transwell inserts for each treatment group were captured using the Inverted Leica DMI8 microscope. White = macrophage nuclei. Scale bar = 100 μm; the concentrations [pg/mL] of (E) IFN-γ, (F) TNF-α, (G) TGF-β, and (H) IL-6 in the intermediate buffer from the Transwell assay were quantified for each treatment group using ELISA. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison test. Data are presented as mean ± SD, with a p -value ≤ 0.05 considered statistically significant.

Article Snippet: The following materials and reagents were used in this study: Membrane Protein Extraction Kit (ProteoExtract), acetone, chloroform, methanol, DMSO, Tween 20, phosphate-buffered saline (PBS), RPMI-1640, DMEM, Eukitt (R) Quick-hardening mounting medium, 25 mm Sterile Syringe Filters, 0.02 μm PVDF, and cholesterol from Sigma-Aldrich-Merck; 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC), 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), and 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine- N -(lissamine rhodamine B sulfonyl) (PE-Rhod) from Avanti Polar Lipids, Inc.; dialysis tubes (12–14 kDa and 1000 kDa) from Repligen; Dynamic Light Scattering (DLS), ZetaSizer Nano, and disposable cuvettes for zeta potential measurements from Malvern Instruments; rabbit anti-CD11b (MAB11242–100) for Western blotting, and DuoSet ELISA kits for TNF-α, TGF-β, IFN-γ and IL-6 from R&D Systems; rabbit anti-CD68 (ab125212), rabbit anti-CD163 (ab182422), goat antirabbit IgG-HRP (ab6721), hematoxylin and eosin kit, and DAB substrate kit from Abcam; flow cytometry antibodies: anti-CD45-FITC (BLG-157608), anti-CD11b-Alexa Fluor 647 (BLG-101218), anti-F4/80-APC-Cy7 (BLG-157315), anti-Ly6G-BV711 (BLG-127643), anti-Ly6C-PE (BLG-128008), anti-CD3-Spark Blue 574 (BLG-100276), anti-CD8a-Alexa Fluor 594 (BLG-100758), anti-CD4-BV510 (BLG-100449), anti-FoxP3-Pacific Blue (BLG-126410), anti-CD19-PE-Cy7 (BLG-152418), and Zombie Violet Viability Kit from BioLegend; semi microvolume disposable polystcytokinesyrene cuvettes for size measurements and MycoStrip Mycoplasma Detection Kit from Tamar Ltd.; 5X Sample Buffer from A2S; Trans-Blot Turbo Mini PVDF membrane, TC20 Automated Cell Counter and slides, and Clarity Western ECL Substrate from Bio-Rad Laboratories; Quick Coating Solution from AngioProteomie; NanoAssemblr Benchtop and Microfluidic Cartridge from Precision Nanosystems (Cytiva); Infinite M Plex multimode microplate reader from Tecan; Pierce BCA kit,1-Step TMB ELISA Substrate Solutions, Vybrant DiD Cell-Labeling Solution, Halt protease inhibitor cocktail, Quant-iT Endotoxin Detection Assay Kit ( Q32892 ) and wheat germ agglutinin-Alexa Fluor 488 from ThermoFisher Scientific; μ-slide 0.4 was purchased from ibidi, and Endothelial Cell Medium with supplements from ScienCell; InVivo mAb antimouse PD-1 (CD279) (BE0146–5A) from BioXCell.

Techniques: Migration, Liposomes, Inhibition, Staining, Microscopy, Transwell Assay, Enzyme-linked Immunosorbent Assay, Comparison